The group attempted to block the arrival of a vessel owned by shipping line ZIM, with union members joining in on the rally.
“To know that innocent people – kids, families, mums and dads – are dying, it’s just not on mate,” Dean Smith from the Maritime Union of Australia said.
The ship itself never entered the port and redirected upon news of the demonstration.
The rally went ahead today despite calls from the government for it to be cancelled.
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Premier Chris Minns said earlier this week that while he welcomes peaceful demonstrations protesters can not stop trade.
Meanwhile a burger shop in Melbourne’s south-east was the alleged target of arsonists early on Friday morning which sparked a clash between Israel and pro-Palestinian supporters on Friday night.
This led to several arrests and forced a nearby synagogue to evacuate.
The organisers issued an apology to the local Jewish community who had their worship affected last night.