A 24-year-old Melton man was among those charged, after being caught without a licence while operating a jet ski on the waterway.
Water Police Acting Sergeant Kristie Clappison said police are concerned about the dangerous behaviour of some individuals.
“People think jet skis are toys,” she told 9News.
“People don’t realise how powerful and heavy jetskis are and what sort of damage they can do.
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“(People) flick others with water, ride too close and sometimes those jet skis become uncontrollable.”
The incident at Mornington Peninsula, which was captured on video by a passenger, allegedly involved the teenager deliberately spraying the occupants of the boat with water before speeding off.
Acting Sergeant Clappison said any illegal behaviour would not be tolerated.
Police will continue to patrol holiday hotspots, beaches and waterways across Victoria.