New research suggests that far from indiscriminate slaughter, human sacrifice in ancient Central America involved the careful selection of victims based on gender, age, and even family.

The ancient city of Chichén Itzá in Yucatán, Mexico, became a dominant Mayan settlement from about 800CE to 1000CE.

Evidence of ritual sacrifices is abundant throughout the site, including the Sacred Cenote, a large sinkhole containing the remains of more than 200 individuals.

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El Castillo, also known as the Temple of Kukulcan, is among the largest structures at Chichén Itzá and its architecture reflects its far-flung political connections. (Johannes Krause)

In 1967, a chultun — an underground cistern or water storage chamber — containing the remains of more than 100 young individuals was discovered near the Sacred Cenote.

Radiocarbon dating indicated the chultun was in use between the early 600s and the mid-1100s CE.

Researchers have now been able to use modern techniques to recover and analyse the DNA of 64 of those individuals found within.

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Detail from the reconstructed stone tzompantli, or skull rack, at Chichén Itzá. (Christina Warinner)
In findings published in the journal Nature, the authors said the genetic analysis revealed that all 64 individuals were male, and about 25 per cent of those analysed were closely related, including two sets of twins.

By contrast, young adult females and male and female children have been found in the Sacred Cenote

Ritualistic sacrifice of children has been speculated to support crop yields and rainfall, the authors note, and twin sacrifice has been associated with Mayan mythology.

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It’s believed that Mayan authorities then preferred young males for their sacrifices at the chultun, while other sacrificial practices were observed in different rites.

The Mayan civilisation is estimated to have taken form in about 250CE, and finally collapsed with the Spanish conquests of the 1500s and 1600s.

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