The family of imprisoned former US Marine Corps pilot and Australian citizen Daniel Duggan has made a fresh plea for his release 1000 days after he was locked up.

The 55-year-old is being held in a maximum security prison in Bathurst, NSW while awaiting extradition to the US.

Former US military pilot and Australian citizen Daniel Duggan. (Supplied Nine)

He was allegedly paid $100,000 for his services.

Duggan has repeatedly denied the accusations.

His wife Saffrine Duggan has called on Prime Minister Anthony Albanese and the Attorney-General Michelle Rowland to release her husband.

“Today my husband and Aussie citizen Dan Duggan has now been locked up in maximum security for 1000 days, with no Australian charges, awaiting extradition to the United States,” she said in a statement.

“Surely that can’t stand in a free, sovereign Australia.

“Please Prime Minister and Attorney General, enough is enough. You need to help your fellow Aussies.”

Daniel Duggan and his wife Saffrine.
Daniel Duggan and his wife Saffrine. (60 Minutes)

Saffrine also thanked the thousands of Australians who continue to have hope by showing their support.

Duggan’s legal team sent a comprehensive 89-page submission to the former Attorney-General in August last year, however he approved Duggan’s extradition in December.

The legal team intends to fight the extradition in the Federal Court, with a date set in August.

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